摘要
      由于大规模城市化，全世界对城市空气质量的担忧日益加剧。大量的工作都集中在空气中的化学污染物及其对人类健康的影响上。然而，空气传播的微生物污染物，特别是抗生素耐药性基因（ARGs），在很大程度上研究不足。本文采用高通量定量PCR（HT-qPCR）对中国厦门市11个不同功能区和不同城市化水平的站点的空气ARGs的时空分布进行了研究。在所有样本中总共检测到104个独特的ARGs和23个可移动遗传元件（MGE）。在ARG剖面的分布中观察到了时间变化，夏季检测到的ARG相对丰度明显高于春季。温度是夏季ARG和MGE总相对丰度的关键预测因子，而PM2.5和PM10是影响春季ARG和MgE丰度的两个最重要因素。我们的研究结果表明，城市气溶胶容纳了丰富而动态的ARGs和MGE，并强调了温度、空气质量和人为活动在形成ARGs分布中的作用。
Abstract
Concerns around urban air quality have been increasing worldwide due to large-scale urbanization. A large volume of work has been focused on the chemical pollutants in the air and their impacts on human health. However, the profile of airborne microbial contaminants, especially antibiotic resistance genes (ARGs), is largely understudied. Here, high-throughput quantitative PCR (HT-qPCR) was employed to explore the temporal and spatial distribution of airborne ARGs from 11 sites with various functional zones and different urbanization levels within Xiamen, China. A total of 104 unique ARGs and 23 mobile genetic elements (MGEs) were detected across all samples. Temporal shift was observed in the distribution of ARG profiles, with significantly higher relative abundance of ARGs detected in summer than that in spring. Temperature is the key predictor of the total relative abundance of ARGs and MGEs in summer, while PM2.5 and PM10 were the two most important factors affecting the abundance in spring. Our findings suggest that urban aerosols accommodate rich and dynamic ARGs and MGEs, and emphasize the role of temperature, air quality and anthropogenic activities in shaping the profile of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722062751