摘要
      本研究调查了沼气残渣和生物炭添加对抗生素抗性基因（ARGs）、移动遗传元件（MGEs）的影响，以及农业垃圾堆肥过程中细菌群落的变化。测序技术研究了细菌群落结构和ARGs、MGE的变化。利用冗余分析确定理化因子、ARGs、MGE和细菌群落结构之间的相关性。结果证实，生物炭和沼渣改良剂有效降低了ARGs和MGEs的含量。检测到的主要ARGs是sul1。变形杆菌和厚壁菌门是与ARGs传播密切相关的主要宿主细菌。细菌群落的动态特征与菌堆温度和pH值密切相关（P<0.05）。冗余度和网络分析表明，硝酸盐、intI1和厚壁菌门主要影响ARGs的变化。因此，调节这些关键变量将有效抑制ARGs的传播和堆肥使用的风险。
Abstract
This research investigated biogas residue and biochar addition on antibiotic resistance genes (ARGs), mobile genetic elements (MGEs), and changes in bacterial community during agricultural waste composting. Sequencing technique investigated bacterial community structure and ARGs, MGEs changes. Correlations among physicochemical factors, ARGs, MGEs, and bacterial community structure were determined using redundancy analysis. Results confirmed that biochar and biogas residue amendments effectively lowered the contents of ARGs and MGEs. The main ARGs detected was sul1. Proteobacteria and Firmicutes were the main host bacteria strongly associated with the dissemination of ARGs. The dynamic characteristics of the bacterial community were strongly correlated with pile temperature and pH (P < 0.05). Redundancy and network analysis revealed that nitrate, intI1, and Firmicutes mainly affected the in ARGs changes. Therefore, regulating these key variables would effectively suppress the ARGs spread and risk of compost use.

https://www.sciencedirect.com/science/article/abs/pii/S0960852423000627