摘要
     探讨了大颗粒尺寸（LPB，5-10 mm）和小颗粒尺寸（SPB，<0.0074 mm）的生物炭在猪粪与玉米秸秆堆肥过程中对抗生素和抗生素抗性基因（ARGs）命运的影响。结果表明，堆肥后对照处理、LPB处理和SPB处理的抗生素去除率分别为40%、50%和76%。SPB的引入使堆肥后的总ARGs和可移动遗传元素（MGE）水平分别降低了28%和19%。Mantel试验结果表明，有机物、水分含量和NH4+-N对抗生素和ARGs的变化贡献最大。生物炭比表面积和抗生素对MGEs的显著影响进一步调节了ARGs的行为。因此，在猪粪堆肥过程中，使用SPB堆肥更有利于抗生素和ARGs的去除。
Abstract
The impact of biochars with large particle sizes (LPB, 5–10 mm) and small particle sizes (SPB, <0.074 mm) on fates of antibiotics and antibiotic resistance genes (ARGs) during composting of swine manure with maize straw was explored. The results showed that antibiotics removal efficiencies were 40 %, 50 %, and 76 % for control treatment, control with LPB treatment, and control with SPB treatment after composting, respectively. The introduction of SPB reduced the total ARGs and mobile genetic elements (MGEs) levels by 28 % and 19 % after composting, respectively. The Mantel test results showed that organic matter, moisture content, and NH4+-N contributed the most to changes in antibiotics and ARGs. The significant effects of biochar specific surface area and antibiotics on MGEs further regulated the behavior of ARGs. Therefore, composting with SPB is more conducive to the removal of antibiotics and ARGs during composting of swine manure.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422018752