摘要
      饮用水处理厂（DWTP）生物滤池中的抗生素耐药性基因（ARGs）被认为是对人类健康的显著潜在风险。一项关于生物过滤器中ARGs的全球调查可能有助于评估其整体风险特征。本研究旨在探讨DWTP生物过滤器中ARGs的组成、风险和生态成因。从国家生物技术信息中心（NCBI）的序列读取档案（SRA）中总共收集了98个DWTP生物过滤器的宏基因组，并识别了主要的ARG类型，其中多药、杆菌肽和β-内酰胺是前三种类型。发现水源水类型（地表水与地下水）会显著影响抗生素耐药性，过度渗透生物过滤器介质和位置。尽管地表水生物滤池的ARG丰度约为地下水生物滤池的五倍，但地表水生物滤器和地下水生物滤器的ARG风险模式高度相似，平均高达99.61%的ARG属于风险最低和未评估等级，只有0.23%属于风险最高等级。在地表水和地下水生物膜样品中，单菌坦生物合成途径和灵菌素生物合成途径这两种抗生素生物合成途径分别与几种ARG类型和总ARG丰度呈正相关，表明它们在ARG的生态成因中具有潜在作用。总的来说，这项研究的结果将加深我们对DWTP生物过滤器中ARG风险的理解，并揭示其内部的生态成因。
Abstract
Antibiotic resistance genes (ARGs) in biofilters of drinking water treatment plants (DWTPs) are regarded to be a remarkable potential health risk to human. A global survey on ARGs in biofilters may help evaluate their risk features as a whole. This study aims to explore the compositions, risks, and ecological genesis of ARGs in the biofilters of DWTPs. In total, 98 metagenomes of DWTP biofilters were collected from Sequence Read Archive  (SRA)  of National center for Biotechnology Information (NCBI), and the main ARG types were recognized, with multidrug, bacitracin, and beta-lactam as the first three types. Source water types (surface water vs. groundwater) were found to significantly influence antibiotic resistome, overpassing biofilter media and locations. Although ARG abundances of surface water biofilters were approximately five times higher than that of groundwater biofilters, the risk pattern of ARGs was highly similar between surface water biofilters and groundwater biofilters, and up to 99.61% of the ARGs on average belong to the least risk and unassessed ranks, and only 0.23% the highest risk rank. Monobactam biosynthesis pathway and prodigiosin biosynthesis pathway, two antibiotics biosynthesis pathways, were observed to be positively correlated with several ARG types and total ARG abundance in samples of surface water and groundwater biofilters, respectively, suggesting their potential roles in ecological genesis of ARGs. Overall, the results of this study would deepen our understanding of the ARG risks in biofilters of DWTPs and shed light on their ecological genesis inside.

https://www.sciencedirect.com/science/article/abs/pii/S0043135423002579