摘要
      沿海泻湖为人类社会提供了许多重要服务，但全年用于水产养殖会产生大量污水。因此，抗生素耐药性基因（ARGs）的污染引起了人们的极大关注。在本研究中，通过高通量定量PCR检测了50个ARGs亚型、两个整合酶基因（intl1、intl2）和16S rRNA基因，并制备了所有靶基因的标准曲线进行定量。对一个典型的滨海泻湖（中国新村泻湖）中ARGs的发生和分布进行了综合探讨。我们在水中和沉积物中分别检测到44种和38种ARGs亚型，并讨论了影响ARGs在沿海泻湖中命运的各种因素。大环内酯类林可沙酰胺类链菌素B是ARG的主要类型，macB是主要的亚型。抗生素外排和抗生素失活是ARG的主要耐药机制。新村泻湖被划分为八个功能区。由于不同功能区的微生物生物量和人为活动的影响，ARGs表现出明显的空间分布。渔排、废弃的鱼塘、城镇污水区和红树林湿地为新村泻湖提供了大量的ARG。营养素和重金属也与ARGs的命运显著相关，尤其是NO2−-N和Cu，这一点不容忽视。值得注意的是，泻湖屏障系统与持久性污染物输入相结合，导致沿海泻湖成为ARGs的“缓冲池”，然后ARGs会积聚并威胁近海环境。
Abstract
Coastal lagoons provide many important services to human society, but their year-round use for aquaculture introduces large amounts of sewage. The contamination of antibiotic resistance genes (ARGs) is therefore of great concern. In this study, 50 ARGs subtypes, two integrase genes (intl1, intl2), and 16S rRNA genes were detected by high-throughput quantitative PCR, and standard curves of all target genes were prepared for quantification. The occurrence and distribution of ARGs in a typical coastal lagoon (XinCun lagoon, China) were comprehensively explored. We detected 44 and 38 subtypes of ARGs in the water and sediment, respectively, and discuss the various factors influencing the fate of ARGs in the coastal lagoon. Macrolides-lincosamides-streptogramins B was the primary ARG type, and macB was the predominant subtype. Antibiotic efflux and antibiotic inactivation were the main ARG resistance mechanisms. The XinCun lagoon was divided into eight functional zones. The ARGs showed a distinct spatial distribution owing to the influence of microbial biomass and anthropogenic activity in different functional zones. Fishing rafts, abandoned fish ponds, the town sewage zone, and mangrove wetlands provided a large quantity of ARGs to the XinCun lagoon. Nutrients and heavy metals also significantly correlated with the fate of the ARGs, especially NO2−-N and Cu, which cannot be ignored. It is noteworthy that lagoon-barrier systems coupled with persistent pollutant inputs result in coastal lagoons acting as a “buffer pool” for ARGs, which can then accumulate and threaten the offshore environment.

https://www.sciencedirect.com/science/article/abs/pii/S1001074222003758