摘要
      抗生素耐药性基因（ARGs）已在环境中被广泛检测到。厌氧消化（AD）具有去除ARGs的潜在能力，需要对AD过程中ARGs变化进行全面研究。在本研究中，研究了上流式厌氧污泥床（UASB）反应器长期运行过程中抗生素抗性基因（ARGs）和微生物群落的变化。向UASB进水中加入红霉素、磺胺甲恶唑和四环素的抗生素混合物，操作期为360天。在UASB反应器中检测了11个ARGs和1类整合子整合酶基因的丰度，并分析了ARGs与微生物群落之间的相关性。ARGs的组成表明，出水中的主要ARGs为sul1、sul2和sul3，而污泥中的主要ARG为tetW。相关分析表明，UASB中的微生物与ARGs呈负相关。此外，大多数ARGs与norank_f_Propionibacteriaceae和Clostridum_senus_stricto_6呈正相关，这两种细菌被确定为潜在宿主。这些发现可能有助于制定一种可行的策略，在厌氧消化过程中去除水生环境中的ARGs。
Abstract
Antibiotic resistance genes (ARGs) have been widely detected in the environment. Anaerobic digestion (AD) has the potential ability to remove ARGs, and a comprehensive study is needed on the variations in ARGs during AD. In this study, variations in antibiotic resistance genes (ARGs) and microbial communities were investigated during the long-term operation of an upflow anaerobic sludge blanket (UASB) reactor. An antibiotic mixture of erythromycin, sulfamethoxazole and tetracycline was added to the UASB influent and the operation period was 360 days. The abundances of 11 ARGs and class 1 integron-integrase gene were detected in the UASB reactor, and the correlation between the ARGs and the microbial community was analyzed. The composition of ARGs indicated that the main ARGs in the effluent were sul1, sul2, and sul3, whereas the main ARG in the sludge was tetW. Correlation analysis indicated a negative correlation between microorganisms and ARGs in the UASB. In addition, most of ARGs showed a positive correlation with norank_f_Propionibacteriaceae and Clostridum_sensu_stricto_6, which were identified as potential hosts. These findings may help develop a feasible strategy for removing ARGs from aquatic environments during anaerobic digestion.

https://www.sciencedirect.com/science/article/abs/pii/S0013935123005479