摘要
      抗生素耐药性病原体和抗生素耐药性基因在环境中的传播对公众健康构成了严重威胁。然而，现有的方法很难有效地从环境中去除抗生素耐药性病原体和ARGs。在本研究中，我们通过重原子效应合成了一种新的吖啶类光敏剂，2,7-二溴-9-甲基-10-甲基高氯酸吖啶（YM-3），该光敏剂可以在水环境中通过产生单线态氧（1O2）来光动力学灭活抗生素抗性病原体并减少ARGs。YM-3的1O2产率是其改性前体的4.9倍。在低强度蓝光（15W/m2）照射2小时和8小时后，YM-3可将耐甲氧西林金黄色葡萄球菌和耐碳青霉烯类鲍曼不动杆菌的可培养数甚至活菌数分别降至0（灭活率>99.99999%）。光照20小时后，两种细菌中ARGs的拷贝数分别减少了5.80和4.48 log，这可能表明ARGs已经被降解。此外，YM-3在经过五个灭活周期后仍具有有效的杀菌作用。YM-3具有超低光强要求和高效杀菌能力等特点，在室内和阳光环境中具有良好的消毒应用前景。
Abstract
The spread of antibiotic resistant pathogens and antibiotic resistance genes (ARGs) in the environment poses a serious threat to public health. However, existing methods are difficult to effectively remove antibiotic resistant pathogens and ARGs from the environment. In this study, we synthesized a new acridine-based photosensitizer, 2,7-dibromo-9-mesityl-10-methylacridinium perchlorate (YM-3), by the heavy atom effect, which could photodynamically inactivate antibiotic resistant pathogens and reduce ARGs by generating singlet oxygen (1O2) in an aqueous environment. The 1O2 yield of YM-3 was 4.9 times that of its modified precursor. YM-3 could reduce the culturable number and even the viable counts of methicillin-resistant Staphylococcus aureus and carbapenem-resistant Acinetobacter baumannii to 0 (inactivation rate > 99.99999%) after 2 and 8 h of low-intensity blue light (15 W/m2) irradiation, respectively. After 20 h of light exposure, the copy numbers of ARGs in both bacteria were reduced by 5.80 and 4.48 log, respectively, which might indicate that ARGs had been degraded. In addition, YM-3 still had an efficient bactericidal effect after five inactivation cycle. These characteristics of ultra-low light intensity requirement and efficient bactericidal ability make YM-3 have good application prospects for disinfection in indoor and sunlight environment.

https://www.sciencedirect.com/science/article/pii/S0160412023001125