摘要
      这项工作研究了基于宏基因组学的抗生素抗性基因（ARGs）及其潜在宿主在微米级零价铁（mZVI）增强的废物活性污泥的嗜热厌氧消化（TAD）过程中的行为和风险。用0、25、100和250 mg mZVI/g总固体（TS）进行测试。结果表明，100mg mZVI/g TS可使ARGs的丰度和多样性分别降低7.3%和4.8%。在这种条件下，具有健康风险的ARGs丰度和人类致病菌（HPB）多样性也分别降低8.3%和3.6%。此外，mZVI随着剂量的增加，将72种潜在致病超级载体的丰度分别降低了2.5%、5.0%和6.1%，用于具有高健康风险的ARGs。总的来说，mZVI，特别是在100 mg/g TS下，可以减轻TAD的抗生素耐药性风险。这些发现对于更好地了解ARGs及其致病宿主在ZVI增强的固体废物TAD中的风险很重要。
Abstract
This work investigated the metagenomics-based behavior and risk of antibiotic resistance genes (ARGs), and their potential hosts during thermophilic anaerobic digestion (TAD) of waste activated sludge, enhanced by micron-scale zero valent iron (mZVI). Tests were conducted with 0, 25, 100, and 250 mg mZVI/g total solids (TS). Results showed that up to 7.3% and 4.8% decrease in ARGs’ abundance and diversity, respectively, were achieved with 100 mg mZVI/g TS. At these conditions, ARGs with health risk in abundance and human pathogenic bacteria (HPB) diversity were also decreased by 8.3% and 3.6%, respectively. Additionally, mZVI reduced abundance of 72 potential pathogenic supercarriers for ARGs with high health risk by 2.5%, 5.0%, and 6.1%, as its dosage increased. Overall, mZVI, especially at 100 mg/g TS, can mitigate antibiotic resistance risk in TAD. These findings are important for better understanding risks of ARGs and their pathogenic hosts in ZVI-enhanced TAD of solid wastes.

https://www.sciencedirect.com/science/article/abs/pii/S0960852423003218