摘要
      随着新出现的污染物，废水中的抗生素和抗生素耐药性基因（ARGs）会诱导并增加抗生素耐药性在环境中传播的风险。在此，我们制备了一系列聚苯胺/碳纳米管辅助电活性膜（PANI/CNT-EMs），以去除低浓度抗生素（即磺胺甲恶唑、SMX）、抗生素耐药菌（ARB）和ARGs，从而降低废水中的生态毒性和抗生素耐药性污染。PANI/CNT EM具有优异的电化学性能，具有均匀稳定的结构。与原始EM相比，PANI/CNT EM有效地增强了SMX的降解，并显著降低了降解产物的生态毒性。PANI/CNT-EMs在+2V的低电压下对截留的ARB显示出更高的失活能力，并且在废水流出物电过滤过程中对细胞内和细胞外ARG（iARGs和eARGs）都表现出显著的降解。更重要的是，减少的整合素（即intI1）丰度和ARG转移频率进一步表明PANI/CNT-EMs更有利于控制抗生素耐药性的传播。此外，与原始EM相比，PANI/CNT EM在废水处理过程中的防污性能提高了42.1%。总的来说，这些发现为废水处理中的抗生素耐药性控制提供了一种很有前途的方法。
Abstract
As emerging contaminants, antibiotic and antibiotic resistance genes (ARGs) in wastewater effluent induce and increase the risk of antibiotic resistance spreading in the environment. Herein, we fabricated a series of polyaniline/carbon nanotube-assisted electroactive membranes (PANI/CNT-EMs) to remove low-concentration antibiotics (i.e., sulfamethoxazole, SMX), antibiotic-resistant bacteria (ARB), and ARGs, thus reducing ecotoxicity and antibiotic resistance pollution in wastewater effluent. The PANI/CNT-EMs possess excellent electrochemical properties with a uniform and stable structure. Compared with pristine EMs, the PANI/CNT-EMs effectively enhanced SMX degradation and substantially lessened the ecotoxicity of the degradation products. The PANI/CNT-EMs show higher inactivation ability in intercepted ARB and significant degradation of both intracellular and extracellular ARGs (iARGs and eARGs) during wastewater effluent electrofiltration under a low voltage of +2 V. More importantly, the reduced integron (i.e., intI1) abundance and ARG transfer frequency further demonstrated that the PANI/CNT-EMs are more conducive to controlling the spread of antibiotic resistance. Furthermore, compared with pristine EMs, the antifouling property of PANI/CNT-EMs is improved by 42.1% during wastewater effluent treatment. Overall, these findings offer a promising approach for antibiotic resistance control in wastewater effluent treatment.

https://www.sciencedirect.com/science/article/abs/pii/S0376738823000856