摘要
      噬菌体可以通过转导促进抗生素抗性基因（ARGs）在农业环境中的传播。然而，尚未对堆肥过程中噬菌体介导的ARG谱进行研究。本研究探讨了在猪粪堆肥过程中添加腐殖酸（HA）对细菌/噬菌体共介导的ARGs（b/pARGs）丰度的影响，以及影响b/pARG传播的关键因素。结果表明，在堆肥过程中添加5%HA可以有效降低b/pARGs的绝对丰度，抑制携带ARGs致病微生物（如棒状杆菌和链球菌）的增殖，并通过调节关键环境因素来改变共宿主细菌的丰度，最终影响b/pARG在堆肥过程的命运。总的来说，本研究的发现为了解影响b/pARGs分布的主要驱动因素提供了新的信息，并为堆肥过程中ARGs污染的预防和控制提供了参考。
Abstract
Phages can promote the spread of antibiotic resistance genes (ARGs) in agricultural environments through transduction. However, studies on phage-mediated ARG profiles during composting have not been performed. This study investigated the effects of adding humic acid (HA) on the abundances of bacteria/phage co-mediated ARGs (b/pARGs) during swine manure composting and the key factors that affected the transmission of b/pARGs. The results showed that the addition of 5 % HA during composting could effectively reduce the absolute abundances of b/pARGs, inhibit the proliferation of pathogenic microorganisms (e.g., Corynebacterium and Streptococcus) that carried ARGs, and ultimately affect the fate of b/pARGs in the composting process by regulating key environmental factors to change the abundance of co-host bacteria. Overall, the findings of this study provided new information for understanding the main driving factors affecting the b/pARGs profile and provided a reference for the prevention and control of ARGs pollution during composting.

https://www.sciencedirect.com/science/article/abs/pii/S0960852423001475