摘要
      抗生素降解菌通常用于治疗抗生素污染，但它们携带的抗生素耐药性基因（ARGs）经常被忽视。本研究使用宏基因组组装和装箱分析来探索猪粪堆肥过程中潜在的抗生素降解细菌及其ARGs。结果表明，35个宏基因组组装基因组（MAGs）主要包含参与抗生素去除的烷基芳基转移酶和脱羧酶基因。多药（1 2 4）、β-内酰胺（67）、大环内酯林可酰胺链菌素（MLS）（64）和四环素（43）是在35个MAG中检测到的中心ARG类型。此外，使用arg_ranker框架评估了ARGs的风险，发现19个MAG含有具有人类相关富集、基因可转移性和宿主致病性的中高风险ARGs。Geofilum属的Bin 34具有最高的ARG风险。Limnochorda属、Chelatococcus属和Niabella属的Bin 6、Bin 11和Bin 14的ARG风险较低，被认为是潜在的抗生素降解细菌。
Abstract
Antibiotic-degrading bacteria are commonly used to treat antibiotic contamination, but the antibiotic resistance genes (ARGs) they carry are often overlooked. This study used metagenomic assembly and binning analyses to explore potential antibiotic-degrading bacteria and their ARGs during pig manure composting. The result showed that 35 metagenome-assembled genomes (MAGs) mainly containing alkyl-aryl transferase and decarboxylase genes involved in the removal of antibiotics. Multidrug (1 2 4), β-lactam (67), macrolide-lincosamide-streptogramin (MLS) (64), and tetracycline (43) were the central ARG types detected in the 35 MAGs. Furthermore, the risk of ARGs was evaluated using the arg_ranker framework, and 19 MAGs were found to contain intermediate-high-risk ARGs with human-associated-enrichment, gene transferability, and host pathogenicity. Bin 34 of the genus of Geofilum had the highest ARG risk. Bin 6, Bin 11 and Bin 14 of the genus of Limnochorda, Chelatococcus and Niabella, had a lower ARG risk and were considered as potential antibiotic-degrading bacteria.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422018739