摘要
      在本研究中，开发了一种将等离子体与过乙酸相结合的新型绿色方法（等离子体/PAA），以同时去除废水中的抗生素和抗生素抗性基因，在去除效率和能量产量方面取得了显著的协同效应。在2.6A的等离子体电流和10mg/L的PAA剂量下，实际废水中大多数检测到的抗生素在2分钟内的去除率超过90%，ARG的去除率在6.3%-75.2%之间。血浆和PAA的协同作用可能与活性物质（包括•OH、•CH3、1O2、ONOO-、•O2-和NO•）的产生有关，这些物质分解抗生素，杀死宿主细菌，并抑制ARG偶联转移。此外，血浆/PAA还改变了ARG宿主菌的贡献和丰度，下调了双组分调控系统的相应基因，从而减少了ARG的繁殖。此外，抗生素的去除与ARGs之间的弱相关性突出了血浆/PAA在同时去除抗生素和ARGs方面值得称赞的表现。因此，本研究提供了一种创新有效的去除抗生素和ARGs的途径，它依赖于血浆和PAA的协同机制以及废水中抗生素和ARG的同时去除机制。
Abstract
In this study, a novel and green method combining plasma with peracetic acid (plasma/PAA) was developed to simultaneously remove antibiotics and antibiotic resistance genes (ARGs) in wastewater, which achieves significant synergistic effects in the removal efficiencies and energy yield. At a plasma current of 2.6 A and PAA dosage of 10 mg/L, the removal efficiencies of most detected antibiotics in real wastewater exceeded 90 % in 2 min, with the ARG removal efficiencies ranging from 6.3 % to 75.2 %. The synergistic effects of plasma and PAA could be associated with the motivated production of reactive species (including •OH, •CH3, 1O2, ONOO-, •O2- and NO•), which decomposed antibiotics, killed host bacteria, and inhibited ARG conjugative transfer. In addition, plasma/PAA also changed the contributions and abundances of ARG host bacteria and downregulated the corresponding genes of two-component regulatory systems, thus reducing ARG propagation. Moreover, the weak correlations between the removal of antibiotics and ARGs highlights the commendable performance of plasma/PAA in the simultaneous removal of antibiotics and ARGs. Therefore, this study affords an innovative and effective avenue to remove antibiotics and ARGs, which relies on the synergistic mechanisms of plasma and PAA and the simultaneous removal mechanisms of antibiotics and ARGs in wastewater.

https://www.sciencedirect.com/science/article/abs/pii/S0304389423006404