摘要
       抗生素耐药性的出现对公众健康构成了严重威胁。关于污水排放对胶州湾沉积物中抗生素和抗生素抗性基因（ARGs）污染的影响的研究很少。本文对胶州湾海洋沉积物中281种ARG亚型、10种可移动遗传元件（MGE）、10种抗生素和细菌群落进行了表征。在海湾内的各个采样点之间发现了类似的细菌群落结构和ARG谱，这些采样点都以多药和（氟喹诺酮）耐药性基因为主，并且ARG的相对丰度低于海湾出口附近采样点的相对丰度。与抗生素和MGE相比，细菌群落组成是ARG多样性和地理分布的更重要驱动因素。受污水排放的影响，胶州湾南部携带遗传信息的病原体数量急剧增加，这表明污水排放促进了海洋沉积物的ARG污染。这项研究强调了将经处理的废水排放到海洋环境中传播抗生素耐药性影响因素的风险。迫切需要优化或改进废水处理工艺，以提高抗生素和ARGs的去除率。这项研究对填补胶州湾抗生素耐药性信息空白和制定未来的污染调控措施具有必要的意义。
Abstract
The emergence of antibiotic resistance is a severe threat to public health. There are few studies on the effects of sewage discharge on antibiotics and antibiotic resistance genes (ARGs) contamination in Jiaozhou Bay sediment. Herein, a total of 281 ARG subtypes, 10 mobile genetic elements (MGEs), 10 antibiotics and bacterial communities in marine sediments from Jiaozhou Bay were characterized. Similar bacterial community structures and ARG profiles were identified between the various sampling sites inside the bay, which were both dominated by multidrug and (fluoro)quinolone resistance genes and harbored lower relative abundances of ARGs than those in the sampling sites near the bay exit. Compared with antibiotics and MGEs, bacterial community composition was a more important driver of ARG diversity and geographic distribution. The abundance of pathogens carrying genetic information increased dramatically in southern Jiaozhou Bay is affected by sewage discharge, which indicating that wastewater discharge facilitated ARG contamination of marine sediments. This study highlights the risk of disseminating antibiotic resistance-influencing factors from treated wastewater discharge into marine environment there is an urgent need to optimize or improve wastewater treatment processes to enhance the removal of antibiotics and ARGs. This study has necessary implications for filling the gap in information on antibiotic resistance in Jiaozhou Bay and developing future pollution regulation and control measures.

https://www.sciencedirect.com/science/article/abs/pii/S0301479723006588