摘要
      高抗生素浓度促进废水中抗生素抗性基因（ARG）的水平基因转移（HGT），同时由于产生抗性而降低ARG的丰度。然而，抗生素浓度对不同抗生素的ARGs丰度的双向影响仍然缺乏。在此，通过在不同浓度下添加两种典型的抗生素（即磺胺嘧啶、SDZ和盐酸四环素、TC-HCl），研究了ARGs的命运和微生物群落的相应组成（即HGT的耐药菌和潜在宿主菌）。结果表明，两种抗生素的初始抗生素浓度对相应ARG（即tet或sul）的丰度有不同的影响；然而，它受到最终抗生素浓度的影响，并且对于两种抗生素治疗，较高的最终抗生素浓度将具有较高的ARG丰度。较高的初始抗生素浓度会降低微生物群落的丰度，但会提高微生物群落的多样性，导致相应抗生素的生物降解能力不同，从而影响微生物群落的抗性，从而改变ARGs（即tet或sul）的丰度。另一方面，更高的最终抗生素浓度具有更高的潜在宿主细菌丰度，从而导致更高丰度的ARGs（即tet或sul）。这项研究为好氧活性污泥反应器中ARG丰度对抗生素浓度选择压力的改变提供了新的见解。
Abstract
A high antibiotic concentration promotes the horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs) in the wastewater while reducing the ARG abundance due to the production of resistance. However, the two-way effects of antibiotic concentration on the abundance of ARGs with different antibiotics are still lacking. Herein, the fate of ARGs and the corresponding composition of the microbial community (i.e., resistome and potential host bacteria for HGT) were investigated by adding two typical antibiotics (i.e., sulfadiazine, SDZ, and tetracycline hydrochloride, TC-HCl) under different concentrations. Results indicated that the initial antibiotic concentration for two antibiotics had different effects on the abundance of the corresponding ARGs (i.e., tet or sul); however, it was affected by the final antibiotic concentration, and a higher final antibiotic concentration would have a higher ARG abundance for both two antibiotic treatments. A higher initial antibiotic concentration would decrease the abundance but improve the diversity of the microbial community, resulting in the different biodegradation abilities of the corresponding antibiotic and subsequently affecting the resistome of microbial community, resulting in altering the abundance of ARGs (i.e., tet or sul). On the other hand, a higher final antibiotic concentration had a higher abundance of potential host bacteria, resulting in a higher abundance of ARGs (i.e., tet or sul). This study provides new insights into the mechanisms underlying the alteration of ARG abundance to the selection pressures of antibiotic concentration in aerobic activated sludge reactors.

https://www.sciencedirect.com/science/article/abs/pii/S2214714423000168