摘要
      洱海是一个典型的高原深水湖，26条支流长期非点源污染排放，对抗性基因的丰度和传播产生了显著影响。在本研究中，对洱海盆地周围的25个抗生素抗性基因（ARGs）进行了调查，分为六种类型，并对NPS污染排放进行了调查。FCA（mexF）和磺酰胺抗性基因（sul1、sul2和sul3）最常见。尽管到目前为止，那里ARGs的绝对总丰度较低，但像磺酰胺抗性基因这样的单个基因却很高。使用普通最小二乘模型（OLS）进行的回归分析表明，向洱海排放NPS污染会对ARGs的分布产生明显影响。它们之间的关系是线性的。具体而言，支流总氮（TN）污染输入与ARG丰度的增加呈显著相关，而总磷（TP）污染输入则呈相反的相关性，并最终影响ARG的分布。此外，TP对ARG分布的影响比TN更显著。本研究提供了云贵高原亚热带深湖ARG分布地理概况。研究结果有利于预测ARGs在高原湖泊中的分布特征和控制其污染。
Abstract
Erhai Lake, a typical plateau deep water lake, experienced long-term nonpoint source (NPS) pollution discharge from 26 tributaries, which significantly affected the abundance and spread of resistance genes. In this study, 25 antibiotic resistance genes (ARGs), classified into six types, and NPS pollution discharges were investigated throughout around the Erhai basin. FCA (mexF) and sulfonamide resistance genes (sul1, sul2 and sul3) were the most common. Although the absolute overall abundance of ARGs there was low so far, the individual gene like sulfonamide resistance gene was high. Regression analysis using an ordinary least squares model (OLS) showed that the discharge of NPS pollution into Erhai Lake would have an obvious effect on the distribution of ARGs. And the relations between them were linear. Concretely speaking, the total nitrogen (TN) pollution input from tributaries could significantly correlated with the increasing of ARG abundance, while the total phosphorus (TP) pollution input showed the opposite correlation, and ultimately affect the distribution of ARGs. Moreover, the effect of TP on ARG distribution was more significant than TN. This study provides a geographical profile of ARG distribution in a subtropical deep lake on Yunnan-Kweichow Plateau. The results are beneficial for predicting the distribution characteristics of ARGs and controlling their pollution in plateau lakes.

https://www.sciencedirect.com/science/article/abs/pii/S0269749122016852