摘要
      为了解决土霉素菌丝体残渣（TMD）的诊断和去除土霉素和抗生素抗性基因（ARGs）的问题，本研究采用好氧堆肥（AC）技术，添加自制复合微生物接种物（HCMI），以促进TMD的AC，提高土霉素和ARGs的去除。研究结果表明，土霉素残留在AC后基本上是无害的。此外，HCMI不仅降低了QacB和tetH，而且使VanRA、VanT和dfrA24的降解率分别提高了40.81%、5.65%和54.18%。HCMI在一定程度上提高了ARG亚型的去除率。根据冗余度分析，在AC期间，微生物群落的演替对ARG亚型的变化的影响比环境条件更强。由于温度变化导致的各种细菌丰度的差异可能是ARG亚型变异的内在机制。
Abstract
In order to tackle the issue of terramycin mycelial dreg (TMD) diagnosis and removal of terramycin and antibiotic resistance genes (ARGs), this study adopted aerobic composting (AC) technology and added homemade compound microbial inoculum (HCMI) to promote the AC of TMD and enhance the removal of terramycin and ARGs. The findings demonstrated that terramycin residue could be basically harmless after AC. Moreover, HCMI not only reduced QacB and tetH but also increased the degradation rates of VanRA, VanT, and dfrA24 by 40.81%, 5.65%, and 54.18%, respectively. The HCMI improved the removal rate of ARG subtypes to a certain extent. According to redundancy analysis, during AC, the succession of the microbial community had a stronger influence on the variance of ARG subtype than the environmental conditions. Differences in the abundance of various bacteria due to changes in temperature may be an intrinsic mechanism for the variation of ARG subtypes.

https://www.sciencedirect.com/science/article/abs/pii/S0960852422016352