摘要
      城市污水处理厂是抗生素抗性基因和致病菌的主要聚集地。在本研究中，应用等离子体氧化来去除废水中的ARGs并抑制其致病性。实验结果表明，在等离子体处理的25分钟内，分别去除了约1.32 log、1.34 log、2.39 log和2.80 log的aac（3）-II、blaTEM-1、TetW和TetC。自由基dotOH、1O2、O3、H2O2、NO2−和NO3−都有助于ARG的去除。共存的溶解有机物也被氧化，这与ARG的去除呈正相关。等离子体氧化导致微生物群落的多样性和丰富度下降；细小杆菌和变形杆菌是ARGs和可移动遗传元件的主要潜在宿主。血浆处理后，整合素基因IntI1下降了2.46 log，这与ARG的去除呈正相关。血浆处理后，与ATP结合、DNA修复、基因转移和细胞膜相关的基因表达减少，从而有效去除ARG。此外，与块状皮肤病、尼曼-匹克病、多囊肾和肝病相关的致病基因都受到血浆治疗的下调。总的来说，血浆处理抑制了ARGs的传播风险和MWTP废水中的致病性。
Abstract
Municipal wastewater treatment plants (MWTP) are the main gathering places for antibiotic resistance genes (ARGs) and pathogenic bacteria. In this study, plasma oxidation was applied to remove the ARGs and inhibit pathogenicity in the effluent. The experimental results demonstrated that approximately 1.32 log, 1.34 log, 2.39 log, and 2.80 log of aac(3)-II, blaTEM-1, TetW, and TetC were removed within 25 min of the plasma treatment, respectively. radical dotOH, 1O2, O3, H2O2, NO2−, and NO3− all contributed to ARG removal. Coexisting dissolved organic matter was also oxidized, which was positively related to ARG removal. Plasma oxidation led to a decrease in the diversity and richness of microbial communities; Parcubacteria and Proteobacteria were the main potential hosts of ARGs and mobile genetic elements. Integron gene IntI1 decreased by 2.46 log after plasma treatment, which was positively related to ARG removal. Gene expression related to ATP binding, DNA repair, gene transfer, and the cell membrane was reduced after plasma treatment, resulting in effective ARG removal. Furthermore, pathogenic genes related to lumpy skin disease, Niemann-Pick disease, polycystic kidney and hepatic disease were all down-regulated by plasma treatment. Overall, the plasma treatment inhibited the transmission risks of ARGs and pathogenicity in the effluent of MWTP.

https://www.sciencedirect.com/science/article/abs/pii/S1385894722057540