摘要
      在本研究中，四环素（TC）可以在微生物燃料电池（MFC）中快速有效地降解，具有微生物代谢和电刺激的协同作用。不同的TC浓度对MFC的生物电性能有不同的影响。其中，10mg/L TC促进了MFC的生物电性能，最大功率密度达到1744.4±74.9mW/cm2。此外，我们证明了地杆菌和Chrysobacterium是阳极生物膜中的优势物种，而Azoarcus和Pseudomonas是出水中的主要物种，初始TC浓度影响微生物群落组成。此外，TC的添加增加了反应器中aadA3、sul1、adeF、cmlA和tetC的相对丰度，表明单一抗生素可以促进自身相关耐药性的表达以及其他ARG的表达。此外，TC的存在会增加可移动遗传元件（MGE）的相对含量，并大大增加抗生素抗性基因（ARGs）传播的风险。同时，网络分析显示，一些微生物（如盲肠不动杆菌、土壤地杆菌和耐热假单胞菌）和MGE可能是多种ARG的潜在宿主。
Abstract
In this study, tetracycline (TC) can be degraded in microbial fuel cells (MFCs) rapidly and efficiently for the synergistic effect of microbial metabolism and electrical stimulation. Different TC concentrations had different effects on the bioelectric performance of MFCs. Among them, 10 mg/L TC promoted the bioelectric properties of MFCs, the maximum power density reached 1744.4 ± 74.9 mW/cm2. In addition, we demonstrated that Geobacter and Chryseobacterium were the dominant species in the anode biofilm, while Azoarcus and Pseudomonas were the prominent species in the effluent, and the initial TC concentration affected the microbial community composition. Furthermore, the addition of TC increased the relative abundance of aadA3, sul1, adeF, cmlA, and tetC in reactors, indicating that a single antibiotic could promote the expression of self-related resistance as well as the expression of other ARGs. Moreover, the presence of TC can increase the relative content of mobile genetic elements (MGEs) and greatly increase the risk of antibiotic resistance genes (ARGs) spreading. Meanwhile, network analysis revealed that some microorganisms (such as Acidovorax caeni, Geobacter soil, and Pseudomonas thermotolerans) and MGEs may be potential hosts for multiple ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0045653522040772