摘要
     粪肠球菌（E.faecalis）中抗生素耐药性基因（ARGs）的迅速传播对人类健康和生态环境安全构成了巨大挑战。因此，控制ARGs的传播是很重要的。在本研究中，我们观察到，添加5μg/mL二十二碳六烯酸（DHA）可使粪便大肠杆菌中pCF10质粒的偶联转移减少95%以上。DHA通过抑制prgZ基因的mRNA水平来干扰信息素的运输，导致iCF10信息素在供体细菌中积累并与PrgX受体结合形成抑制期，这导致与偶联转移相关的基因表达下调，抑制生物膜的形成，减少细菌粘附，从而抑制结合转移。总的来说，DHA对粪便中ARGs的转移表现出令人钦佩的抑制作用。这项研究为控制ARGs的转移提供了一个技术选择。
Abstract
The rapid spread of antibiotic-resistance genes (ARGs) in Enterococcus faecalis (E. faecalis) poses a great challenge to human health and ecological and environmental safety. Therefore, it is important to control the spread of ARGs. In this study, we observed that the addition of 5 μg/mL docosahexaenoic acid (DHA) reduced the conjugative transfer of pCF10 plasmid by more than 95% in E. faecalis. DHA disturbed the pheromone transport by inhibiting the mRNA levels of the prgZ gene, causing the iCF10 pheromone to accumulate in the donor bacteria and bond to the PrgX receptor to form an inhibitory phase, which resulted in the down-regulation of the expression of genes related to conjugative transfer, inhibiting biofilm formation, reducing bacterial adhesion and thus inhibiting conjugative transfer. Collectively, DHA exhibited an admirable inhibitory effect on the transfer of ARGs in E. faecalis. This study provided a technical option to control the transfer of ARGs.

https://www.sciencedirect.com/science/article/abs/pii/S0304389422021847