摘要
      饮用水分配系统（DWDS）中生物膜生长引起的抗生素耐药性基因（ARGs）的传播对人类健康构成了严重威胁。消毒副产物（DBPs）对DWDS不同阶段（生物膜和水）中ARGs和可移动遗传元件（MGE）分布的影响尚不清楚。在本研究中，检测了模拟DWDS反应器运行150天期间生物膜和流出物中的微生物指数、三卤代甲烷（THMs）、卤代乙酸（HAAs）、ARGs和MGEs的特征。在90天或120天的成熟生物膜期内，对于生物膜和废水，七类ARGs（四环素除外）和MGE在整个操作周期中分别处于最高和最低的相对丰度。DBP对DWDS中ARGs和MGE的影响在不同阶段是不同的。在废水中，与MGE相比，DBP与ARGs的关系更强。THMs和HAAs与各种ARGs呈正相关，表明DBPs影响抗性基因的分布。在生物膜中，MGE对ARGs的影响比DBP对ARGs。因此，DBP可能是水中ARGs富集和扩散的重要因素。
Abstract
The spread of antibiotic resistance genes (ARGs) caused by biofilm growth in drinking water distribution systems (DWDSs) poses a serious threat to human health. The influence of disinfection by-products (DBPs) on the distribution of ARGs and mobile genetic elements (MGEs) in different phases (biofilms and water) of DWDSs is unclear. In this study, the characteristics of microbial indices, trihalomethanes (THMs), haloacetic acids (HAAs), ARGs and MGEs in biofilms and effluents of simulated DWDS reactors were detected during 150 d of operation. During the mature biofilm periods of 90 d or 120 d, for both biofilms and effluents, seven categories of ARGs (except tetracycline) and MGEs were at their highest and lowest relative abundance in the entire operation cycle, respectively. The influence of DBPs on ARGs and MGEs in the DWDS was different in different phases. In effluents, DBPs showed a stronger relationship with ARGs compared with MGEs. THMs and HAAs had positive correlations with various ARGs, which indicated that DBPs affected the distribution of resistance genes. In biofilms, the influence of MGEs on ARGs was stronger than that of DBPs on ARGs. Thus, DBPs might be an important contributor to the enrichment and spread of ARGs in water.

https://www.eeer.org/journal/view.php?number=1439