摘要
      海水养殖沉积物一直是抗生素抗性基因（ARGs）的交换和繁殖来源。然而，还没有产生有效的方法来去除沉积物中的ARGs。在这里，我们探讨了过氧化氢（H2O2）和曝气对海水养殖沉积物中ARGs和可移动遗传元素（MGEs）有效去除的影响。与曝气组相比，在前14天，H2O2组的ARG丰度降低了3.8–32.3%。ARG和MGE的丰度也与细菌总数和多样性的减少显著相关（P < 0.05）。基于偏平方路径模型，MGEs的减少在H2O2处理的ARG去除中起着重要作用，而在曝气组中，ARG的减少主要由细菌群落组成的变化决定。这些结果表明，H2O2处理是一种很有前途的方法，可以在给料后控制ARG的丰度，并限制ARG在水产养殖环境中的传播。
Abstract
Mariculture sediments have been exchange and propagation sources of antibiotic resistance genes (ARGs). However, no efficient methods have been generated to remove ARGs from sediments. Here, we explored the impact of hydrogen peroxide (H2O2) and aeration on the efficient removal of ARGs and mobile genetic elements (MGEs) in mariculture sediments. When compared with the aeration group, the ARG abundance was 3.8–32.3% lower in the H2O2 group during the first 14 days. ARG and MGE abundances were also significantly associated with reduced total bacterial population and diversity (P < 0.05). Based on partial squares path modeling, reduction of MGEs had important roles in ARG removal from H2O2 treatments, while in the aeration group, ARG reductions were mainly determined by changes in bacterial community composition. These results suggested that H2O2 treatment represent a promising method for controlling ARG abundance after dosing feed stuff and limit the spread of ARGs in aquaculture environments.

https://www.sciencedirect.com/science/article/abs/pii/S0269749123006541