摘要
      根际是“一个健康”情景的一个极其重要的组成部分，它将土壤微生物组和植物联系起来，其中抗生素抗性基因（ARGs）的潜在富集可能最终流入人类食物链。尽管土壤传播疾病的发生率增加，这可能导致杀虫剂和抗生素生产生物控制剂的使用增加，但对ARG在根际传播动力学的理解在很大程度上被忽视了。在此，选择生长在有利于和抑制病原体青枯菌的土壤中的番茄幼苗作为模型，研究ARG在有病原体入侵和没有病原体入侵的情况下在根际的传播。宏基因组学数据显示，青枯菌的入侵增加了ARGs和移动遗传元件（MGE）的密度。尽管我们在两种土壤中都发现了源自人类致病菌的ARGs，但在抑制性土壤中，这种富集现象有所缓解。总之，抑制性土壤通过抑制病原体阻碍了ARG的传播，并且具有较低数量的抗生素抗性类群。
Abstract
The rhizosphere is an extremely important component of the “one health” scenario by linking the soil microbiome and plants, in which the potential enrichment of antibiotic resistance genes (ARGs) might ultimately flow into the human food chain. Despite the increased occurrence of soil-borne diseases, which can lead to increased use of pesticides and antibiotic-producing biocontrol agents, the understanding of the dynamics of ARG spread in the rhizosphere is largely overlooked. Here, tomato seedlings grown in soils conducive and suppressive to the pathogen Ralstonia solanacearum were selected as a model to investigate ARG spread in the rhizosphere with and without pathogen invasion. Metagenomics data revealed that R. solanacearum invasion increased the density of ARGs and mobile genetic elements (MGEs). Although we found ARGs originating from human pathogenic bacteria in both soils, the enrichment was alleviated in the suppressive soil. In summary, the suppressive soil hindered ARG spread through pathogen suppression and had a lower number of taxa carrying antibiotic resistance.

https://www.sciencedirect.com/science/article/pii/S2352771423000010