摘要
      抗生素废水不仅含有传统污染物，还含有高浓度的抗生素及其产生的污染物，如抗生素抗性微生物和抗生素抗性基因（ARGs）。如果处理不当，抗生素废水将进一步诱导抗生素耐药性微生物和ARGs在环境中的传播。本研究采用上流式厌氧污泥床（UASB）-缺氧/好氧（膜生物反应器）工艺（A/O（MBR））处理土霉素（OTC）废水。结果表明，当OTC浓度从25mg/L增加到50mg/L时，OTC的去除率从72.92%下降到59.60%。当OTC浓度从0增加到50 mg/L时，化学需氧量（COD）的去除率从97.47%下降到95.05%。然而，总氮去除率从55.53%上升到85.33%。拟杆菌和氮螺旋菌分别在UASB和A/O（MBR）中占主导地位，在50 mg/L OTC时相对丰度分别为20.76%和18.51%。UASB和O（MBR）中的ARGs均随着OTC浓度的升高而增加，在50mg/L OTC时分别达到3.44×109和7.61×108拷贝/mL。相应的移动基因元件（MGE）分别达到4.29×108和3.58×108拷贝/mL。该膜具有有效的污染物去除效率，对ARGs和MGEs的截留率分别达到90.86%和87.91%。
Abstract
Antibiotic wastewater contains not only conventional pollutants but also high concentrations of antibiotics and the resulting pollutants, such as antibiotic resistant microorganisms and antibiotic resistant genes (ARGs). If not properly treated, the antibiotic wastewater will further induce the spread of antibiotic resistant microorganisms and ARGs in the environment. In this study, an up-flow anaerobic sludge bed (UASB)-anoxic/aerobic (membrane bioreactor) process (A/O(MBR)) was built to treat oxytetracycline (OTC) wastewater. Results showed that the removal efficiencies of OTC decreased from 72.92% to 59.60% when the OTC concentration increased from 25 mg/L to 50 mg/L. Increasing OTC concentration from 0 to 50 mg/L, the chemical oxygen demand (COD) removal efficiencies decreased from 97.47% to 95.05%. Nevertheless, the total nitrogen removal efficiency increased from 55.53% to 85.33%. Bacteroides and Azospira dominated in UASB and A/O(MBR) respectively with the relative abundance of 20.76% and 18.51% at 50 mg/L OTC. Both ARGs in the UASB and O(MBR) increased with the rise of OTC concentration and reached 3.44 × 109 and 7.61 × 108 copies/mL respectively at 50 mg/L OTC. And the corresponding mobile gene elements (MGEs) reached 4.29 × 108 and 3.58 × 108 copies/mL. The membrane presented an effective pollutants removal efficiency with the interception efficiencies for ARGs and MGEs reaching 90.86% and 87.91%, respectively.

https://www.sciencedirect.com/science/article/abs/pii/S2213343723004384