摘要
      该研究的目的是从二次出水中大量减少兼性致病菌（FPB）和临床相关的抗生素耐药性基因（ARGs）。为了评估ARGs的去除效率，对单个单元工艺和组合AOP进行了比较研究。本工作研究了：i）去除选定的ARGs，即blaTEM、ermB、qnrS、tetM、五个FPB以及16S rDNA和参与水平基因转移的整合酶基因intl1；ii）处理后的细菌再生潜力；（iii）在真实的二级流出物中，在可见光辐射下，通过单金属和双金属TiO2-CuPd金红石光催化剂去除总基因组DNA含量。通过紫外-可见光谱、XRD、HRTEM、拉曼光谱和XPS对纳米颗粒进行了表征。与单金属催化剂相比，TiO2 CuPd/VIS失活对几乎所有靶标（blaTEM、ermB、tetM、intl 1和16S rRNA）都具有最高（超过2个对数单位的去除）效率。在臭氧氧化和TiO2-CuPd/O3/VIS（分别为3.22和3.23LRV）之后实现了对blaTEM的最高效率。研究发现，尽管FPB失活相似，但与TiO2 CuPd/O3/VIS处理相比，O3处理后的DNA破坏更低。在TiO2-CuPd/O3/Vis和TiO2-Cu/O3/Vis过程中，blaTEM和肠球菌被完全去除。而催化臭氧化仅导致轻微的blaTEM去除（TiO2 CuPd/O3和TiO2 Cu/O3的LRV分别为0.64和0.44）。由于Pd和Cu之间的协同作用，应用TiO2-CuPd进行光催化臭氧化可显著去除FPB和ARGs，从而加速臭氧-催化剂反应。光催化臭氧氧化具有最大的潜力，为消除AMR提供了有希望的可能性。
Abstract
The aim of the study was the strong reduction of facultative pathogenic bacteria (FPB), and clinically relevant antibiotic resistance genes (ARGs) from secondary effluent. To evaluate the ARGs removal efficiency comparative study of individual unit processes and combined AOPs has been performed. The present work investigated: i) removal of selected ARGs, namely blaTEM, ermB, qnrS, tetM, five FPB as well as 16S rDNA and the integrase gene intl1 involved in horizontal gene transfer; ii) bacterial regrowth potential after treatment; (iii) the removal of the total genomic DNA content, by mono- and bimetallic TiO2-CuPd rutile photocatalysts under visible light radiation in real secondary effluent. The nanoparticles were characterized by UV–vis, XRD, HRTEM, Raman and XPS. TiO2-CuPd/VIS inactivation gives the highest (more than 2 log units removal) efficiency for almost all targets (blaTEM, ermB, tetM, intl 1, and 16S rRNA) compared to monometallic catalysts. The highest efficiency toward blaTEM was achieved after ozonation and TiO2-CuPd/O3/VIS (3.22 and 3.23 LRV, respectively). It was found that despite similar FPB inactivation, lower DNA destruction was found after O3 compared to TiO2-CuPd/O3/VIS treatment. The blaTEM and enterococci were completely removed during TiO2-CuPd/O3/Vis and TiO2-Cu/O3/Vis. While, catalytic ozonation leads to only slightly blaTEM removal (0.64 and 0.44 LRV for TiO2-CuPd/O3 and TiO2-Cu/O3, respectively). Photocatalytic ozonation with the application of TiO2-CuPd leads to significant removal of FPB and ARGs due to synergistic effect between Pd and Cu, which leads to acceleration of ozone – catalysts reaction. Photocatalytic ozonation has the greatest potential giving promising possibilities for eliminating AMR.

https://www.sciencedirect.com/science/article/pii/S1385894723009749