摘要
      循环水产养殖系统（RAS）可以有效地用于水产养殖，氧化处理通常用于改善水质。然而，氧化处理对RAS中水产养殖水安全和鱼类产量的影响尚不清楚。在本研究中，我们测试了O3和O3/UV处理对养殖鲫鱼水质和安全的影响。O3和O3/UV处理使溶解有机碳（DOC）浓度降低了约40%，并破坏了难降解的有机木质素样特征。在O3和O3/UV处理后，氨氧化细菌（硝化螺旋菌、硝化单胞菌和硝化单胞杆菌）和反硝化细菌（Pelomonas、Methylsovertilis和鞘氨醇单胞菌）富集，氮循环功能基因分别富集23%和48%。O3和O3/UV处理降低了RAS中的NH4+-N和NO2--N。O3/UV处理增加了鱼的长度和重量以及鱼肠道中的益生菌。然而，高饱和中间体和单宁样特征在O3和O3/UV处理中分别诱导了52%和~28%的抗生素抗性基因（ARGs），并增强了ARGs的水平转移。总体而言，O3/UV的应用取得了较好的效果。然而，了解ARGs在RAS中造成的潜在生物风险，并确定最有效的水处理策略来减轻这些风险，应该是未来工作的目标。
Abstract
Recirculating aquaculture systems (RASs) can be efficiently used for aquaculture, and oxidation treatment is commonly used to improve water quality. However, the effects of oxidation treatments on aquaculture water safety and fish yield in RASs are poorly understood. In this study, we tested the effects of O3 and O3/UV treatments on aquaculture water quality and safety during culture of crucian carp. O3 and O3/UV treatments reduced the dissolved organic carbon (DOC) concentration by ∼40% and destroyed the refractory organic lignin-like features. There was enrichment of ammonia oxidizing (Nitrospira, Nitrosomonas, and Nitrosospira) and denitrifying (Pelomonas, Methyloversatilis, and Sphingomonas) bacteria, and N-cycling functional genes were enriched by 23% and 48%, respectively, after O3 and O3/UV treatments. Treatment with O3 and O3/UV reduced NH4+-N and NO2–-N in RASs. O3/UV treatment increased fish length and weight as well as probiotics in fish intestine. However, high saturated intermediates and tannin-like features induced antibiotic resistance genes (ARGs) in O3 and O3/UV treatments, by 52% and ∼28%, respectively, and also enhanced horizontal transfer of ARGs. Overall, the application of O3/UV achieved better effects. However, understanding the potential biological risks posed by ARGs in RASs and determining the most efficient water treatment strategies to mitigate these risks should be goals of future work.

https://www.sciencedirect.com/science/article/abs/pii/S0043135423002701