摘要
      水生环境中抗生素耐药性细菌（ARB）的失活和抗生素耐药性基因（ARGs）的损伤已成为全球控制抗生素耐药性快速传播的主要问题。在本研究中，将带电碳纳米管膜（ECM）与氯消毒（表示为ECM-NaClO系统）相结合，处理水样中的磺酰胺抗性细菌（HLS-6）和四环素抗性细菌（TOP-10F）。ECM NaClO系统通过破坏细胞膜和增加细胞内活性氧（ROS）的数量，有效地灭活了HLS-6和TOP-10F。此外，废水中磺酰胺抗性基因（sul1）和四环素抗性基因（tetA）的绝对丰度分别降低了3.5和1.7 log。此外，可移动遗传元素（intI1）的绝对丰度下降了4.1–4.7个对数。ROS探针化合物降解和猝灭实验表明，通过2-电子-氧还原反应产生的•OH在sul1基因的损伤中起主导作用，而通过NaClO激活产生的•O2–是tetA基因损伤的关键因素。因此，ECM NaClO系统是一种潜在的灭活ARB和破坏水和污水处理厂中ARG的方法。
Abstract
The inactivation of antibiotic-resistant bacteria (ARB) and damage of antibiotic resistance genes (ARGs) in aquatic environments have become major concerns worldwide in controlling the rapid spread of antibiotic resistance. In this study, an electrified carbon nanotube membrane (ECM) was combined with chlorine disinfection (denoted as ECM-NaClO system) to treat the sulphonamide resistance bacteria (HLS-6) and tetracycline resistance bacteria (TOP-10F) in water samples. The ECM-NaClO system effectively inactivated the HLS-6 and TOP-10F by damaging the cell membrane and increasing amounts of intracellular reactive oxygen species (ROS). In addition, the absolute abundances of the sulphonamide resistance gene (sul1) and tetracycline resistance gene (tetA) in the effluent decreased by 3.5 and 1.7 logs, respectively. Furthermore, the absolute abundance of mobile genetic elements (intI1) decreased by 4.1–4.7 logs. The ROS probe compound degradation and quenching experiments revealed that the •OH generated via the 2-electron oxygen reduction reaction played a dominant role in the damage of sul1 gene, while •O2– generated through the activation of NaClO was a key contributor to the damage of tetA gene. Hence, the ECM-NaClO system is a potential method to inactivate ARB and damage ARGs in water and sewage treatment plants.

https://www.sciencedirect.com/science/article/abs/pii/S1385894722056637