摘要
      抗生素抗性基因（ARGs）在土壤、水和空气界面的分配和迁移在抗生素抗性的环境传播中起着关键作用。本研究研究了作为细胞外ARGs（eARGs）代表的抗性质粒在人工构建的土壤-水-空气系统中的分配和迁移。此外，通过正交实验定量研究了土壤pH、粘土矿物含量、有机质含量和模拟降雨对eARGs迁移的影响。研究结果表明，eARGs和土壤之间的吸附平衡可以在3小时内达到，遵循两室一级动力学模型。eARGs在土壤、水和空气中的平均分配比为7:2:1，土壤pH值和粘土矿物含量被确定为主要影响因素。eARGs从土壤迁移到水和空气的比例分别为8.05%和0.52%。相关性和显著性分析表明，土壤pH值对eARGs的土壤水分和土壤空气流动性有显著影响，而粘土含量影响迁移过程中峰值的百分比。此外，降雨对迁徙过程中的高峰时间产生了显著影响。本研究对eARGs在土壤、水和空气中的比例提供了定量的见解，并从吸附机制的角度阐明了影响eARGs分配和迁移的关键因素。
Abstract
The partitioning and migration of antibiotic resistance genes (ARGs) at the interfaces of soil, water, and air play a critical role in the environmental transmission of antibiotic resistance. This study investigated the partitioning and migration of resistant plasmids as representatives of extracellular-ARGs (eARGs) in artificially constructed soil-water-air systems. Additionally, it quantitatively studied the influence of soil pH, clay mineral content, organic matter content, and simulated rainfall on the migration of eARGs via orthogonal experiments. The findings revealed that the sorption equilibrium between eARGs and soil can be attained within 3 h, following the two-compartment first-order kinetic model. The average partition ratio of eARGs in soil, water, and air is 7:2:1, and soil pH and clay mineral content are identified as the main influencing factors. The proportion of eARGs migrating from soil to water and air is 8.05% and 0.52%, respectively. Correlation and significance analyses showed that soil pH has a significant impact on the soil-water and soil-air mobility of eARGs, while clay content affects the percentage of peaks during migration. Moreover, rainfall exerts a noticeable impact on the timing of peaks during migration. This study provided quantitative insights into the proportion of eARGs in soil, water, and air and elucidated the key factors influencing the partitioning and migration of eARGs from the perspectives of the sorption mechanism.

https://www.sciencedirect.com/science/article/abs/pii/S0269749123005596