摘要
      本研究采用高通量荧光定量PCR技术，分析了典型农艺措施和土壤类型对土壤抗生素抗性基因（ARGs）的耦合效应。结果表明，农艺措施提高了不同土壤类型ARG的丰度。对于相同的土壤类型，ARG丰度因农艺措施而增加的顺序如下：施用牲畜粪便（257.6–131.7%）>污水灌溉（57.6–35.0%）>地膜覆盖（21.2–5.0%）。在相同的农艺措施下，ARG丰度随土壤类型的增加依次为：粘土（257.6–21.2%）>壤土（147.8–15.9%）>砂质（131.7–5.0%）。此外，环境因素在决定不同土壤类型ARG丰度方面的贡献因不同农艺措施的应用而不同。邻苯二甲酸盐对沙质土壤和壤土中ARG丰度的影响分别最大和最小。在壤土中，水分对ARG丰度的影响较小，但在粘土和砂土中影响较大。这些发现为通过农艺措施促进土壤ARGs的传播提供了新的见解。
Abstract
In this study, field-scale experiments were carried out to analyze the coupling effects of typical agronomic measures and soil types on soil antibiotic resistance genes (ARGs) using high-throughput fluorescence quantitative PCR. The results indicated that agronomic measures enhanced ARG abundance in different soil types. For the same soil type, the enhancement of ARG abundance in response to agronomic measures could be ordered as follows: livestock manure application (257.6–131.7 %) > sewage irrigation (57.6–35.0 %) > plastic film mulching (21.2–5.0 %). Under the same agronomic measures, enhancement of ARG abundance according to soil type was: clay (257.6–21.2 %) > loam (147.8–15.9 %) > sandy (131.7–5.0 %). Furthermore, the contributions of environmental factors in determining ARG abundance in different soil types differed in response to the application of different agronomic measures. Phthalates had the most and least pronounced effects on ARG abundance in sandy and loam soils, respectively. Effects of moisture on ARG abundance were minor in loam soil, but greater in clay and sandy soils. These findings provide new insights into the dissemination of soil ARGs promoted by the adoption of agronomic measures.

https://www.sciencedirect.com/science/article/abs/pii/S0929139323000355