摘要
      采用固相萃取-超高效液相色谱-串联质谱（SPE-UPLC-MS/MS）和实时定量PCR技术，分析了武汉市16个水源地13种抗生素和10种抗生素抗性基因的分布特征、相关性和潜在的生态风险。分析了该地区抗生素和耐药基因的分布特征、相关性和潜在的生态风险。结果显示，在16个水源样品中共检测到9种抗生素，浓度范围为ND-177.36 ng·L-1。浓度分布如下：支流聚水＜长江干流下游＜长江干流上游＜支流汉江＜支流射水。长江和汉江汇流后ARGs的总绝对丰度显著高于汇流前，磺胺ARGs平均丰度显著高于其他三个ARGs抗性基因（P<0.05）。ARGs中sul1和sul2、ermB、qnrS、tetW和intI1之间存在显著正相关（P<0.01），相关系数分别为0.768、0.648、0.824、0.678和0.790。磺酰胺类ARGs组内的相关性较弱。各组间ARG的相关性。磺胺甲恶唑、金霉素、罗红霉素和恩诺沙星四种抗生素对水生敏感生物具有中等风险，生态风险图中每个风险区域的比例分别为9.0%（中等风险）、30.6%（低风险）和60.4%（无风险）。16个水源地的综合生态风险评估（RQsum）显示为中等风险，涉及河流的RQsum（平均值）为0.222（汉江支流）<0.267（长江干流）<0.299（其他支流）。
Abstract 
The distribution characteristics, correlations, and potential ecological risks of 13 antibiotics and 10 antibiotic resistance genes (ARGs) in 16 water sources in Wuhan were analyzed using solid-phase extraction-ultra-high performance liquid chromatography-tandem mass spectrometry (SPE-UPLC-MS/MS) and real-time quantitative PCR technology. The distribution characteristics and correlations and potential ecological risks of antibiotics and resistance genes in this region were analyzed. The results showed that a total of nine antibiotics were detected in the 16 water source samples, and the concentration range was ND-177.36 ng·L-1. The concentration distribution presented as follows:Tributary Jushui River

https://europepmc.org/article/med/37040953