摘要
      污水处理厂作为抗生素的“来源”和“汇”，含有丰富的抗生素抗性基因和抗生素抗性细菌。在本研究中，调查了位于中国北部城市（保定）的六个城市和两个农村污水处理厂中五种典型ARG的分布、环境相关性和微生物群落。结果表明，在所有样品中，TetC和sulⅢ是主要的ARGs，平均为7.21×106，1.5×106拷贝/mL。Pearson相关分析表明，ARGs与TN、TP和NH4+密切相关。此外，ARGs和细菌属之间的共生模式揭示了活性污泥中ARGs的14个潜在宿主细菌属，这可能促进抗性基因的传播和转移。这项研究全面阐明并强调了采取科学策略控制ARGs传播和改善ARGs消除的必要性。
Abstract
Sewage treatment plants, as the "source" and "sink" of antibiotics, contained abundant antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria. In this study, it was investigated the distribution of five typical ARGs, environmental correlation, and microbial community in six urban and two rural sewage treatment plants, located at the northern city of China (Baoding). The results showed that TetC and sulⅢ were the predominant ARGs in all samples with average 7.21×106, 1.5×106 copies/mL. Pearson correlation analysis showed that ARGs were closely correlation with TN, TP and NH4+. Furthermore, the co-occurrence patterns between ARGs and bacterial genera revealed 14 potential hosts bacterial genera for ARGs in activated sludge, which might promote the spread and transfer of resistance genes. This study provided comprehensive clarify and highlight the necessity for scientific strategies to control ARGs propagation and improve ARGs elimination.

https://www.researchsquare.com/article/rs-2567640/v1