摘要
目标
本研究的目的是探索来自英国菌株的坏死梭杆菌的抗微生物耐药性基因决定簇和表型抗生素易感性数据。研究了在公开可获得的组装全基因组序列中检测到的抗微生物耐药性基因进行比较。
方法
从冷冻瓶（Prolab）中复活了三百八十五株坏死隐球菌（1982–2019）。在测序（Illumina）和质量检查之后，374个全基因组可用于分析。使用BioNumerics（bioMérieux；v8.1）询问基因组是否存在已知的抗微生物耐药性基因（ARGs）。还检查了313个坏死隐球菌分离株（2016-2021）的琼脂稀释敏感性结果。
后果
313个当代菌株的表型数据显示，使用EUCAST v 11.0断点的三个分离株和使用v 13.0分析的73个（23%）菌株对青霉素具有潜在耐药性。除克林霉素（n=2）外，所有菌株都对v11.0指导下的多种药物敏感。使用v 13.0断点，还检测到甲硝唑（n=3）和美罗培南（n=13）的耐药性。tet（O）、tet（M）、tet（40）、aph（3'）-III、ant（6）-la和blaOXA-85 ARGs存在于公开的基因组中。在英国菌株中发现了tet（M）、tet（32）、erm（A）和erm（B），相应地提高了克林霉素和四环素的最低抑制浓度。
结论
不应假设对推荐用于治疗坏死性隐球菌感染的抗生素的易感性。有证据表明ARG可能通过口腔细菌传播，并在坏死隐球菌中检测到转座子介导的β-内酰胺酶耐药性决定簇，必须继续并加强对表型和基因型抗菌药物易感性趋势的监测。
Abstract
Objectives
The objective of the study was to explore antimicrobial resistance gene determinant, and phenotypic antibiotic susceptibility, data for Fusobacterium necrophorum from a collection of UK strains. Antimicrobial resistance genes detected in publicly available assembled whole genome sequences were investigated for comparison.

Methods
Three hundred and eighty five F. necrophorum strains (1982–2019) were revived from cryovials (Prolab). Subsequent to sequencing (Illumina) and quality checking, 374 whole genomes were available for analysis. Genomes were interrogated, using BioNumerics (bioMérieux; v 8.1), for the presence of known antimicrobial resistance genes (ARGs). Agar dilution susceptibility results for 313 F. necrophorum isolates (2016–2021) were also examined.

Results
The phenotypic data for the 313 contemporary strains demonstrated potential resistance to penicillin in three isolates, using EUCAST v 11.0 breakpoints, and 73 (23%) strains using v 13.0 analysis. All strains were susceptible to multiple agents using v 11.0 guidance other than clindamycin (n = 2). Employing v 13.0 breakpoints, metronidazole (n = 3) and meropenem (n = 13) resistance were also detected. The tet(O), tet(M), tet(40), aph(3’)-III, ant(6)-la and blaOXA-85 ARGs were present in publicly available genomes. tet(M), tet(32), erm(A) and erm(B) were found within the UK strains, with correspondingly raised clindamycin and tetracycline minimum inhibitory concentrations.

Conclusions
Susceptibility to antibiotics recommended for the treatment of F. necrophorum infections should not be assumed. With evidence of potential ARG transmission from oral bacteria, and the detection of a transposon-mediated beta-lactamase resistance determinant in F. necrophorum, surveillance of both phenotypic and genotypic antimicrobial susceptibility trends must continue, and increase.

https://www.sciencedirect.com/science/article/abs/pii/S1075996423000264