摘要
      四环素（TC）是一种抗生素，最近被发现是一种新出现的具有低生物降解性的污染物。生物降解显示出TC耗散的巨大潜力。在本研究中，从活性污泥和土壤中分别富集了两个降解TC的微生物群落（分别命名为SL和SI）。与原始微生物群相比，这些最终富集的群落中的细菌多样性降低。此外，在驯化过程中量化的大多数ARG在最终富集的微生物群落中变得不那么丰富。16S rRNA测序显示，这两个群落的微生物组成在一定程度上相似，优势属假单胞菌属、鞘氨醇杆菌属和无色杆菌属被确定为潜在的TC降解菌。此外，群落SL和SI在7天内对TC（初始50 mg/L）的生物降解能力分别为82.92%和86.83%。它们可以在宽pH范围（4−10）和中/高温（25–40°C）下保持高降解能力。浓度为4–10 g/L的蛋白胨可以作为群落通过共代谢去除TC的理想初级生长基质。在TC降解过程中，共检测到16种可能的中间体，包括一种新型生物降解产物TP245。宏基因组测序显示，过氧化物酶基因、tetX样基因和与芳香化合物降解相关的富集基因可能是TC生物降解的原因。
Abstract
Tetracycline (TC) is an antibiotic that is recently found as an emerging pollutant with low biodegradability. Biodegradation shows great potential for TC dissipation. In this study, two TC-degrading microbial consortia (named SL and SI) were respectively enriched from activated sludge and soil. Bacterial diversity decreased in these finally enriched consortia compared with the original microbiota. Moreover, most ARGs quantified during the acclimation process became less abundant in the finally enriched microbial consortia. Microbial compositions of the two consortia as revealed by 16 S rRNA sequencing were similar to some extent, and the dominant genera Pseudomonas, Sphingobacterium, and Achromobacter were identified as the potential TC degraders. In addition, consortia SL and SI were capable of biodegrading TC (initial 50 mg/L) by 82.92% and 86.83% within 7 days, respectively. They could retain high degradation capabilities under a wide pH range (4−10) and at moderate/high temperatures (25–40 °C). Peptone with concentrations of 4–10 g/L could serve as a desirable primary growth substrate for consortia to remove TC through co-metabolism. A total of 16 possible intermediates including a novel biodegradation product TP245 were detected during TC degradation. Peroxidase genes, tetX-like genes and the enriched genes related to aromatic compound degradation as revealed by metagenomic sequencing were likely responsible for TC biodegradation.

https://www.sciencedirect.com/science/article/abs/pii/S0304389423002662