摘要
      受采矿影响的环境分布在全球，并越来越被认为是抗生素耐药性基因（ARGs）的热点。然而，目前还没有关于处理技术来处理如此重要的环境问题的报告。为了缩小这一知识差距，我们在一个酸性铜矿尾矿库中实施了一个植物稳定项目，并利用宏基因组学来探索土壤样本中的ARG特征。我们的研究结果表明，植物稳定使0–10 cm土层中的总ARG丰度降低了75%，同时ARG迁移率显著降低，ARG多样性和微生物多样性显著增加。植物稳定性也被发现会显著改变ARG宿主的组成，并显著降低ARG宿主毒力因子基因的总丰度。土壤养分状况、重金属毒性和SO42-浓度是影响ARG总丰度的重要物理化学因素，而因果中介分析表明，它们的影响在很大程度上是由ARG迁移率和微生物多样性的变化介导的。与植物稳定相关的ARG多样性的增加主要是由一小群ARG宿主介导的，其中大多数不能在属水平上分类，值得未来进一步研究。
Abstract
Mining-impacted environments are distributed globally and have become increasingly recognized as hotspots of antibiotic resistance genes (ARGs). However, there are currently no reports on treatment technologies to deal with such an important environmental problem. To narrow this knowledge gap, we implemented a phytostabilization project in an acidic copper mine tailings pond and employed metagenomics to explore ARG characteristics in the soil samples. Our results showed that phytostabilization decreased the total ARG abundance in 0–10 cm soil layer by 75 %, which was companied by a significant decrease in ARG mobility, and a significant increase in ARG diversity and microbial diversity. Phytostabilization was also found to drastically alter the ARG host composition and to significantly reduce the total abundance of virulence factor genes of ARG hosts. Soil nutrient status, heavy metal toxicity and SO42- concentration were important physicochemical factors to affect the total ARG abundance, while causal mediation analysis showed that their effects were largely mediated by the changes in ARG mobility and microbial diversity. The increase in ARG diversity associated with phytostabilization was mainly mediated by a small subgroup of ARG hosts, most of which could not be classified at the genus level and deserve further research in the future.

https://www.sciencedirect.com/science/article/abs/pii/S0304389422020490