摘要
      良性前列腺增生（BPH）是老年男性的常见疾病。我们通过高通量RNA测序分析获得了前列腺增生症的转录特征。因此，我们确定了前列腺增生组织和正常前列腺组织之间的差异表达RNA（DERNAs）。使用WebGestalt和R软件包（clusterprofiler）进行富集分析。使用斯皮尔曼系数分析临床相关性。TargetScan、ENCORI、miRNet和miRDB数据库用于预测ceRNA网络中靶标的关系。进行免疫荧光染色和qRT-PCR分析以验证研究结果。数据集的微阵列分析显示369个DElncRNA、122个DEpseudogenes、6个DEmiRNA和1358个DEmRNA。DEmRNA在自噬相关途径中特别富集。在筛选DEmRNA和自噬相关基因（ARGs）后，选择了50个DEARGs。对50个DEARG在Cytoscape上进行MCODE分析，获得3个枢纽基因（ATF4、XBP1和PPP1R15A）。Spearman相关性分析显示，XBP1的mRNA表达与年龄、总分和储存分数呈正相关，但与最大流速呈负相关。随后，鉴定了假基因/lncRNA-hsa-miR-222-3p-XBP1通路。我们的研究结果表明，假基因/lncRNA-hsa-miR-222-3p-XBP1通路可能通过自噬在前列腺增生的发生中发挥调节作用。
Abstract
Benign prostatic hyperplasia (BPH) is a common disease among aging males. We obtained BPH transcriptional signatures by high-throughput RNA sequencing analysis. Accordingly, we determined the differentially expressed RNAs (DERNAs) between BPH tissues and normal prostate tissues. WebGestalt and R package (clusterprofiler) was used to enrichment analysis. Clinical correlations were analyzed using Spearman's coefficient. TargetScan, ENCORI, miRNet, and miRDB databases were used to predict targets' relationships in ceRNA networks. Immunofluorescence staining and qRT-PCR analyses were performed to validate the findings. Microarray analysis of the datasets showed 369 DElncRNAs, 122 DEpseudogenes, 6 DEmiRNAs and 1358 DEmRNAs. DEmRNAs were particularly enriched in the autophagy-related pathways. Following the screening of DEmRNAs and autophagy-related genes (ARGs), 50 DEARGs were selected. MCODE analysis on Cytoscape was performed for the 50 DEARGs, and 3 hub genes (ATF4, XBP1, and PPP1R15A) were obtained. Spearman's correlation analysis showed that the mRNA expression of XBP1 correlated positively with age, total score, and storage score, but negatively with the maximum flow rate. Subsequently, the pseudogene/lncRNA- hsa-miR-222-3p-XBP1 pathway was identified. Our findings elucidate that the pseudogene/lncRNA-hsa-miR-222-3p-XBP1 pathway may play a regulatory role in the occurrence of BPH through autophagy.

https://www.sciencedirect.com/science/article/abs/pii/S0141813022027210