摘要

这项研究的目的是评估一种生物可降解薄膜的有效性，其中掺入了Lactobacillus curvatus P99产生的抗微生物代谢产物，目的是控制切片“Prato”奶酪中的单核细胞增生李斯特氏菌。进行测试以评估P99的无细胞上清液（CFS）针对不同微生物的作用谱，以及检测针对单核细胞增生利斯特氏菌ScottA的最小抑制浓度（MIC）和杀菌（MBC）浓度。检测进行编码细菌素产生和评估其表达的基因。制备抗微生物膜，然后进行体外和原位分析。 CFS对抗单核细胞增生利斯特氏菌Scott A的MIC和MBC分别为15.6μL/ mL和62.5μL/ mL。 curvatus P99呈现出两种编码细菌素的基因，它们被表达。添加MBC的膜显示出针对不同指示微生物的活性，并且当用于切片“Prato”奶酪时能够控制单核细胞增生利斯特氏菌Scott A。

The aim of this study was to evaluate the effectiveness of a biodegradable film, with antimicrobial metabolites produced by Lactobacillus curvatus P99 incorporated, targeting the control of Listeria monocytogenes in sliced “Prato” cheese. Tests were performed to evaluate the spectrum of action of cell-free supernatant (CFS) of P99 against different microorganisms, as well as to detect the minimum inhibitory (MIC) and bactericidal (MBC) concentrations against L. monocytogenes Scott A. The detection of genes that encode for the production of bacteriocins and evaluation of their expression were performed. Antimicrobial films were prepared, followed by in vitro and in situ analysis. The MIC and MBC of CFS against L. monocytogenes Scott A was 15.6 μL/mL and 62.5 μL/mL, respectively. Lactobacillus curvatus P99 presented two genes coding for the bacteriocins, which were expressed. Films with added MBC showed activity against different indicator microorganisms and were able to control L. monocytogenes Scott A when used in sliced “Prato” cheese.

https://www.sciencedirect.com/science/article/pii/S0740002016305330