摘要
      废水处理厂（WWTP）是公认的生物气溶胶来源，可能是抗生素抗性细菌和抗生素抗性基因（ARGs）的热点。生物气溶胶可能是细菌和ARGs扩散到环境中的来源。生物过滤是缓解污水处理厂气味的最有效技术之一。本研究的目的是评估气味生物过滤系统的能力，该系统旨在去除挥发性化合物，影响空气中细菌的多样性，并降低雾化微生物和ARG的浓度。在内部污水处理厂处理前后总共采集了28个空气样本。总体而言，上游采集的空气样本的细菌总浓度较高，并观察到细菌多样性的变化。在低丰度的上游和下游检测到军团菌和分枝杆菌，而在两个样本中检测到嗜肺军团菌，但无法量化。在定量聚合酶链式反应检测到的31种ARGs和可移动遗传元件中，有15种在生物过滤后其相对丰度显著降低，没有一种在出水中显著升高。总的来说，这些结果表明了气味生物过滤系统在降低处理过的空气中的细菌和抗微生物耐药性方面的优势，这是限制环境扩散的一个很有前途的应用。
Abstract
Wastewater treatment plants (WWTPs) are confirmed sources of bioaerosols and can be a hotspot for both antibiotic-resistant bacteria and antibiotic-resistant genes (ARGs). Bioaerosols can be a source of dispersion for bacteria and ARGs into the environment. Biofiltration is one of the most effective technologies to mitigate odors from WWTPs. The objective of this study was to evaluate the capacity of an odor biofiltration system designed to remove volatile compounds, to influence the airborne bacterial diversity and to reduce the aerosolized microbial and ARG concentrations. In total, 28 air samples were collected before and after treatment of an interior WWTP. Overall, air samples collected upstream had higher total bacterial concentrations, and a shift in bacterial diversity was observed. Legionella and Mycobacterium were detected in low abundance upstream and downstream, whereas Legionella pneumophila was detected but not quantifiable in two samples. Of the 31 ARGs and mobile genetic elements detected by quantitative polymerase chain reaction, 15 exhibited a significant reduction in their relative abundance after biofiltration, and none were significantly higher in the effluent. Overall, these results show the benefits of odor biofiltration systems to reduce bacterial and antimicrobial resistance in treated air, a promising application to limit environmental dispersion.

https://www.mdpi.com/2073-4433/13/10/1723