摘要
      本研究的目的是寻找APG的最佳用量和初始pH值，以加速废活性污泥（was）的水解并促进抗生素的释放。在本研究中，研究了APG用量为0.1-0.5g/gSS和初始pH为4.0-12.0对WAS水解的影响。定期从不同反应条件下采集样品，以测定SCODcr、蛋白质、多糖、抗生素、抗生素抗性基因（ARGs）和微生物多样性。结果表明，APG+pH的联合作用极大地促进了有机物的增溶，并促进了抗生素的释放。与未处理的污泥相比，当APG的最佳剂量为0.3g/gSS，pH为11.0时，有机物的释放显著增加，SCODcr、蛋白质和多糖的浓度在2小时内分别达到5650.4、1386.4和794.2mg/L。同时，抗生素（四环素类、磺酰胺类、喹诺酮类和大环内酯类）也从固相转移到液相，其中罗红霉素（ROM）比未治疗的对照组高72.6倍，因此四环素ARGs-tetB比对照组高774.2倍。同时，APG大大降低了WAS样品中的微生物多样性，并大大增加了蛋白细菌的相对丰度，其中γ-变形菌是主要的微生物。
Abstract
The purpose of this study was to find the optimal dosage of APG and initial pH to accelerate the hydrolysis of the waste activated sludge (WAS) and promote the release of antibiotics. In this study, the effects with APG dosage from 0.1 to 0.5 g/gSS and initial pH from 4.0 to 12.0 on WAS hydrolysis were investigated. Samples were regularly collected from different reaction conditions to determine SCODcr, protein, polysaccharide, antibiotics, antibiotic resistance genes (ARGs) and microbial diversity. The results showed that the combined action of APG+pH greatly promoted the solubilization of organic matter, and promoted the release of antibiotics. Compared with untreated sludge, when the optimal APG dosage was 0.3 g/gSS with pH 11.0 was combined, the release of organic matter was significantly increased, and the concentrations of the SCODcr, protein and polysaccharide reached 5650.4, 1386.4 and 794.2 mg/L, respectively within 2 h. At the same time, antibiotics (tetracyclines, sulfonamides, quinolones and macrolides) was also transferred from solid phase to liquid phase, among them roxithromycin (ROM) was 72.6 times higher than that in untreated control group, accordingly, the tetracycline ARGs tetB was 774.2 times higher than that in the control group. Meanwhile, APG greatly reduced the microbial diversity in the WAS samples, and greatly increased the relative abundance of proteobacteria, in which Gammaproteobacteria was the main microorganism.

https://www.sciencedirect.com/science/article/abs/pii/S2213343722004250