摘要
      抗生素耐药性基因（ARGs）是一类新的环境污染物，对人类和环境都有危害。到目前为止，经济有效地消除ARG仍然是一个挑战。在本研究中，将光催化技术与人工湿地相结合来去除ARGs，这可以去除细胞内和细胞外的ARGs并降低抗性基因传播的风险。本研究包括三个装置：系列光催化处理人工湿地（S-PT-CW）、人工湿地光催化处理（B-PT-CW）和单一人工湿地（S-CW）。结果表明，光催化和CWs共同提高了去除ARGs的效率，尤其是细胞内ARGs（iARGs）。iARGs去除的对数值在1.27到1.72之间，而eARGs去除率的对数值仅为0.23到0.65。iARG的去除效率排序为B-PT-CW>S-PT-CW>S-CW，对细胞外ARGs（eARGs）的去除效率排名为S-PT-CW>B-PT-CW>S-CW。对S-PT-CW和B-PT-CW去除机理的进一步研究表明，CW是去除iARG的主要途径，而光催化是去除eARG的首要途径。纳米TiO2的加入改变了水煤浆中微生物的多样性和结构，导致脱氮除磷微生物的丰度增加。靶ARGs（sul1、sul2和tetQ）的主要潜在宿主是弧菌属、葡糖杆菌属、链球菌属、梭杆菌属和盐单胞菌属；从废水中去除这些潜在的宿主可能是由于它们的丰度降低。
Abstract
Antibiotic resistance genes (ARGs) is a new class of environmental pollutants that endanger both humans and the environment. So far, removing ARGs economically and efficiently has remained a challenge. In this study, combining photocatalytic technology with constructed wetlands (CWs) were used to remove ARGs, which can remove both intracellular and extracellular ARGs and reduce the risk of resistance gene spread. This study includes three devices: a series photocatalytic treatment-constructed wetland (S-PT-CW), a built photocatalytic treatment into a constructed wetland (B-PT-CW), and a single constructed wetland (S-CW). Results demonstrated that photocatalysis and CWs together increased the efficiency of removing ARGs, particularly intracellular ARGs (iARGs). The log values of iARGs removal ranged from 1.27 to 1.72, while the log values of eARGs removal were only 0.23–0.65. The iARG removal effectiveness was ranked as B-PT-CW > S-PT-CW > S-CW, and the removal effectiveness for extracellular ARGs (eARGs) was ranked as S-PT-CW > B-PT-CW > S-CW. Further investigation into the removal mechanisms of S-PT-CW and B-PT-CW revealed that CWs represented primary pathways for iARG removal, whereas photocatalysis was the primary pathways for eARG removal. The addition of nano-TiO2 altered the diversity and structure of the microorganisms in CWs, leading to an increase in the abundance of nitrogen and phosphorus removal microorganisms. The main potential hosts for target ARGs (sul1, sul2 and tetQ), were the genera Vibrio, Gluconobacter, Streptococcus, Fusobacterium, and Halomonas; the removal of these potential hosts from wastewater may result from their decreased abundance.

https://www.sciencedirect.com/science/article/abs/pii/S0045653523005970