摘要
      Acesulfame钾（ACE-K）是一种广泛使用的代糖，需求量越来越大，由于其顽固性，在各种环境基质中经常被检测到。然而，关于硝化和反硝化过程对去除ACE-K的贡献，目前还缺乏普遍共识。因此，在接种相同活性污泥的硝化序批式反应器（N-SBR）和反硝化序批反应器（D-SBR）中，研究了ACE-K的去除及其对抗生素抗性基因（ARGs）繁殖和微生物群落的影响。在本研究中，经过13次驯化后，N-SBR中的ACE-K可以被去除，去除率达到令人满意的水平（96.76±8.33%），而d-SBR中的平均ACE-K去除率在84天内保持不变（2.24±1.86%）。此外，ACE-K几乎不影响这两种反应器的性能，对硝化和反硝化功能基因的影响也很小。然而，最初接触ACE-K会增加ARGs的丰度，网络分析表明，每个反应器中的功能细菌都可能是ARGs宿主。在两个反应器中都发现了潜在的ACE-K降解属Chelatococcus、Bosea和Aquamibium。LefSe分析表明，含有水微生物属的Phyllobacteriaceae是N-SBR中的一个差异富集家族。这项研究可能为更好地理解废水处理过程中影响ACE-K命运的因素及其生态风险提供一个视角。
Abstract
Acesulfame potassium (ACE-K) is a widely utilized sugar substitute with increasing demand, which is frequently detected in various environmental matrix due to recalcitrance. However, a general consensus on the contribution of nitrifying and denitrifying process to ACE-K removal is lacking. Therefore, ACE-K removal, its effects on antibiotic resistant genes (ARGs) propagation and microbial community in nitrifying sequencing batch reactor (N-SBR) and denitrifying sequencing batch reactor (D-SBR) inoculated with the identical activated sludge were investigated. In this study, ACE-K can be eliminated in N-SBR with satisfying removal efficiency (96.76 ± 8.33 %) after 13 d acclimation, while it remained persistent (average ACE-K removal efficiency of 2.24 ± 1.86 %) in D-SBR during 84 d exposure. Moreover, ACE-K hardly affected the performances of these two types of reactors and had little impact on nitrifying and denitrifying functional genes. However, initial contact with ACE-K would increase ARGs abundance, network analysis showed functional bacteria in each reactor were possible ARGs hosts. Potential ACE-K degrading genera Chelatococcus, Bosea and Aquamicrobium were found in both reactors. LefSe analysis showed that Phyllobacteriaceae containing Aquamicrobium genus was a differentially enriched family in N-SBR. This research might provide a perspective for better understanding factor affecting ACE-K fate in wastewater treatment process and its ecological risks.

https://www.sciencedirect.com/science/article/abs/pii/S0048969722063379