摘要
      抗生素抗性基因（ARGs）被定义为新的环境污染物，在污水处理厂的二次出水中经常检测到，已成为对人类健康和生态安全的潜在威胁。在本研究中，采用慢速过滤和低压纳滤（NF）相结合的工艺从二级出水中彻底去除ARGs和溶解有机碳（DOC）。此外，还讨论了ARGs的去除效率，并分析了组合工艺对膜污染机理的影响。结果表明，生物膜-慢滤-NF联合工艺可以有效降低二级出水中的ARGs和DOC。四种ARGs（tet A、tet W、sul I和sul II）的去除量在4.83-5.67-log范围内，DOC的去除率为82.5%。水中存在的四种ARG与微生物总数、整合素intI1和DOC浓度呈显著正相关，验证了上述指标的去除有助于减少不同类型的ARGs。与直接过滤和慢速过滤相比，生物膜慢速过滤的膜比通量下降速度最低，膜污染指数最小。膜污染特性曲线还表明，生物膜-慢滤-纳滤联合工艺表现出温和的膜污染。总的来说，慢速过滤-NF组合工艺被认为是去除ARG的更好工艺。
Abstract
Antibiotic resistance genes (ARGs) have been defined as new environmental pollutants, which have been frequently detected in secondary effluent from wastewater treatment plants and have become a potential threat to human health and ecological security. In this study, the combined process of slow filtration and low-pressure nanofiltration (NF) was used to thoroughly remove ARGs and dissolved organic carbon (DOC) from secondary effluent. In addition, the removal efficiency of ARGs was discussed and the influence of the combined process on the membrane fouling mechanism was analyzed. The results showed that the combined biofilm slow filtration–NF process can effectively reduce ARGs and DOC in the secondary effluent. The removal amount of the four ARGs (tet A, tet W, sul I, and sul II) was in the range of 4.83- to 5.67-log and the removal rate of DOC was 82.5%. Four ARGs present in the water had a significant positive correlation with the total number of microorganisms, integron intI 1, and DOC concentration, validating that the removal of the above indicators could help reduce different types of ARGs. Compared with the direct NF and slow filtration–NF, the rate of decline of the membrane specific flux of biofilm slow filtration–NF was the lowest, with a minimal membrane fouling index. The membrane fouling characteristic curve also showed that the combined biofilm slow filtration–NF process exhibited mild membrane fouling. Overall, the combined slow filtration–NF process is considered to be a better process for removing ARGs.

https://www.liebertpub.com/doi/abs/10.1089/ees.2021.0587