摘要
      氟沙星（OFL）是农村污水中一种典型的高浓度、高环境风险的抗生素，具有较高的生物活性和抗菌降解能力。本文建立了一个基于厌氧折流板反应器和综合人工湿地的农村污水联合处理设施，并对OFL和常规污染物的去除性能、途径和机理进行了评价。结果表明，OFL和TN的去除率分别达到91.78±3.93%和91.44±4.15%。污泥吸附是OFL的主要去除途径。宏基因组学分析表明，变形杆菌对OFL的去除至关重要。baca是主要的抗生素抗性基因（ARGs）。此外，高丰度的碳代谢有助于OFL的解毒，从而提高系统的稳定性和性能。共生网络分析进一步阐明互惠共生是微生物的主要生存模式。反硝化菌Microbacterium、Geobacter和Ignavibacterium是ARGs的宿主，参与了OFL的生物降解。
Abstract
Ofloxacin (OFL) with high biological activity and antimicrobial degradation is a kind of the typical high concentration and environmental risk antibiotics in rural sewage. In this paper, a combined rural sewage treatment facility based on anaerobic baffled reactor and integrated constructed wetlands was built and the removal performance, pathway and mechanism for OFL and conventional pollutants were evaluated. Results showed that the OFL and TN removal efficiency achieved 91.78 ± 3.93 % and 91.44 ± 4.15 %, respectively. Sludge adsorption was the primary removal pathway of OFL. Metagenomics analysis revealed that Proteobacteria was crucial in OFL removal. baca was the dominated antibiotic resistance genes (ARGs). Moreover, carbon metabolism with a high abundance was conductive to detoxify OFL to enhance system stability and performance. Co-occurrence network analysis further elucidated that mutualism was the main survival mode of microorganisms. Denitrifers Microbacterium, Geobacter and Ignavibacterium, were the host of ARGs and participated in OFL biodegradation.

https://www.sciencedirect.com/science/article/abs/pii/S0960852423000378