摘要

      在本研究中，使用SBR工艺在60天内研究了砷对磺胺甲恶唑（SMX）去除效率和微生物群落结构的影响。结果表明，砷的存在对系统性能没有显著影响，R1（对照试验）和R2（添加砷）两个反应器的去除率分别为13.36±5.71和14.20±5.27%，这归因于胞外聚合物中的黄腐酸样物质和色氨酸样蛋白对SMX的吸附。与种子污泥相比，物种数量表明，在第60天，R2具有更丰富的多样性，而R1具有较低的多样性。这可能与污泥细菌群落中抗生素抗性基因（ARGs）的转移有关；微量砷可以使R2中编码磺酰胺ARGs的Sul1和Sul2基因的相对水平（2.07%和2.47%）高于R1中的相对水平，这使得R2系统的细菌群落更能适应SMX胁迫。因此，微量砷削弱了SMX对系统的影响，增强了微生物群落结构的稳定性。In this study, the effect of arsenic on the sulfamethoxazole (SMX) removal efficiency and microbial community structure was investigated over 60 days using the SBR process. The results showed that the presence of arsenic had no significant impact on the system performance, the removal efficiencies of two reactors, R1 (the control test) and R2 (with the addition of arsenic), were 13.36 ± 5.71 and 14.20 ± 5.27%, which were attributed to the adsorption of SMX by fulvic acid-like substances and tryptophan-like proteins of extracellular polymeric substances. Compared to the seed sludge, the species number indicated that R2 possessed the richer diversity, while R1 possessed the lower diversity on day 60, which might be relative to the transferring of antibiotic resistance genes (ARGs) in sludge bacterial communities; the minute amounts of arsenic could make the relative levels of Sul1 and Sul2 genes which encode ARGs of sulfonamides in R2 (2.07 and 2.47%) be higher than that in R1 (1.65 and 1.27%), which made the bacterial community of the R2 system more adaptable to SMX stress. Therefore, the minute amounts of arsenic weakened the effect of SMX on the system and enhanced the stability of the microbial community structure.

https://iwaponline.com/wst/article/87/2/423/93022/Effect-of-minute-amounts-of-arsenic-on-the