摘要
      这项工作的目的是通过宏基因组学分析，确定高级氧化系统对哥伦比亚波哥大污水处理厂（“El Salitre”WWTP）废水中病原微生物分类和功能多样性的影响。通过Illumina质谱测定氧化处理前后微生物的分类和功能多样性，检测出假单胞菌属（28.0%）、Arcobacter属（10.0%）、Aeromonas属（4.0%）、硫螺旋菌属（4.0%-）、沙门氏菌属（3.0%）和梭菌属（3.0%-）。同样，在高丰度（约占宏基因组读数的5%）中也检测到致病因子，如抗微生物耐药性基因（ARGs）。在用光电Fenton系统处理的流出物中（5、30和60分钟），在所有情况下都观察到致病因子的显著降低。致病菌（如Arcobacter、Pseudomonas、Serratia和Salmonella）的ARGs、趋化性、群体感应、分泌系统和毒素在接近零log2值时减少，这表明光电芬顿过程是一种很有前途的替代方法，可以降低与该致病因子相关的生物风险。
Abstract
The objective of this work was to determine the effect of advanced oxidation systems on the taxonomic and functional diversity of pathogenic microorganisms in the effluent of the wastewater treatment plant in Bogotá - Colombia (“El Salitre” WWTP) by metagenomics analysis. The taxonomic and functional diversity of the microorganisms, before and after oxidation treatment, was determined by Illumina mass sequencing, detecting human pathogenic genera as Pseudomonas (28.0%), Arcobacter (10.0%), Aeromonas (4.0%), Sulfurospirillum (4.0%), Salmonella (3.0%), and Clostridiales (3.0%). Likewise, pathogenicity factors such as antimicrobial resistance genes (ARGs) also were detected in high abundances (~5% of metagenome reads). In the effluent treated with the photoelectro-Fenton system (for 5, 30, and 60 minutes), a significant reduction of pathogenicity factors was observed in all cases. ARGs, chemotaxis, quorum sensing, secretion systems, and toxins of pathogenic bacteria (such as Arcobacter, Pseudomonas, Serratia, and Salmonella) were reduced at near-zero log2 values, demonstrating that the photoelectro-Fenton process is a promising alternative to reduce the biological risk associated with this pathogenicity factors.

https://www.sciencedirect.com/science/article/abs/pii/S2213343723006760