摘要
      由于抗生素的广泛使用，环境中微生物对抗生素的耐药性日益增加，抗生素耐药性基因（ARGs）问题越来越严重，严重威胁生态安全和人类健康。为了研究ARGs和微生物群落在长江口沿海地区不同介质中的分布特征，通过实地调查收集了8个地点的水和沉积物样本。检测并分析了两个磺酰胺抗性基因（[STBX]sul1，sul2[STBZ]）和六个四环素抗性基因（tetM，tetC，tetX，tetA，tet0，和tetQ），一个整合酶基因intI[STBX]1[STBZ]，16S rRNA基因和微生物群落。结果表明，长江口沿海地区10个抗性基因的检出率为100%。[STBX]intI1[STBZ]与水样中的各种ARGs呈显著正相关。变形菌门和拟杆菌门是长江口水环境中的优势菌门。叶绿体是水体中的主要细菌属，叶绿体和硝化螺旋菌是沉积物中的主要菌属。在水中，硝基螺菌是四个四环素抗性基因（tetX、tetA、tetO和tetQ）的共同潜在宿主。在沉积物中，Sva0485是[STBX]sul1和intI1[STBZ]共享的潜在宿主群落。微生物群落的分布是影响ARGs在长江口近岸海域迁移转化的重要因素。
Abstract 
Due to the extensive use of antibiotics, the resistance of microorganisms to antibiotics in the environment is increasing, and the problem of antibiotic resistance genes (ARGs) is becoming more and more severe, which seriously threatens ecological security and human health. In order to study the distribution characteristics of ARGs and the microbial community in different media in the coastal area of the Yangtze River Estuary, water and sediment samples from eight sites were collected through a field investigation. Two sulfonamide resistance genes ([STBX]sul1, sul2[STBZ]) and six tetracycline resistance genes (tetM, tetC, tetX, tetA, tetO, and tetQ), one integrase gene intI[STBX]1[STBZ], 16S rRNA gene, and the microbial community were detected and analyzed. The results showed that the detection rate of 10 resistance genes in the coastal area of the Yangtze River Estuary was 100%. [STBX]intI1[STBZ] was significantly positively correlated with various ARGs in the water samples. Proteobacteria and Bacteroidota were the dominant bacteria phyla in the water environment of the Yangtze River Estuary. Chloroplast was the main bacteria genus in water, and Chloroplast and Nitrospira were the main bacteria genera in sediment. In water, Nitrospirota was the common potential host of four tetracycline resistance genes (tetX, tetA, tetO, and tetQ). In sediments, Sva0485 was a potential host community shared by [STBX]sul1 and intI1[STBZ]. The distribution of the microbial community was an important factor affecting the migration and transformation of ARGs in the nearshore area of the Yangtze River Estuary.

https://europepmc.org/article/med/36635804